ALT淀粉酶活性单位位U/L怎么转换成ng/ml

HCG的单位ng/ml和mol/ml是怎么换算的_百度宝宝知道关注今日:37 | 主题:515082
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【求助】急求100U/ml human IL-2换算成ng/ml是多少?
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本人买的R&D公司human IL-2 10ug/vial。查文献所需要的IL-2量均为U/ml,查了很多资料有说不能换算的,还有说IU和重量之间的换算要看试剂本身来定。。。。现在急求是否能换算?不胜感激~
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林小沫 本人买的R&D公司human IL-2 10ug/vial。查文献所需要的IL-2量均为U/ml,查了很多资料有说不能换算的,还有说IU和重量之间的换算要看试剂本身来定。。。。现在急求是否能换算?不胜感激~是的,这个要看你产品本身生产出来后有没有测活过,而且不同批号的往往还不同 的网站上有certificate of analysis,你输入你的批号应该就能查询到活性单位的信息。
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计算方法参考下面:How can I convert your product to international units?R&D Systems intentionally sells most proteins by mass rather than units. The commonly accepted definition is that 1 unit is equal to the ED50 in a given bioassay. However, varying assay conditions can affect the ED50. Therefore, to follow a protocol given in units or to compare a commercially available product sold in units, one must verify how the unit was defined. It is strongly recommended that a dose response be done when beginning experiments, or with new lots or vendors. Our recommendation is using the midpoint of our ED50 range as the midpoint in the titration, and also running samples at 5, 10, 20, or 50 fold increments above and below this point. If the author/vendor has compared their &unit& to a WHO standard unit, you may find information contained in the WHO Conversion Table on our website to determine roughly where to start. This table contains side-by-side comparison data for those proteins that have a WHO standard available. The WHO standards are available from the NIBSC for purchase and can be used side by side in your own assay as well. R&D提供的换算表 1 ?g =1000ng=1.3 x 104 U1ng=13U (需要多少ng,用U除以13就可以了)同时正如
站友 所说的,不同批次可能会活性会不同,要注意一下。recombinant human IL-2( rhIL-2) 截图
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Debio edited on
换算介绍。看看<font style="color:#IU = 1/22 mg<font style="color:# IU =
ng<font style="color:# IU = 4.54545.... x 10^4 ng.: 1 IU/ml = 4.54545.... x 10^4 ng/ml
国外一个帖子
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建议你直接问销售公司。
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楼主后来怎么换算的啊,我也需要呢
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楼主后来怎么换算的啊,我也需要呢
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