Applied&and&Environmental&Microbiology办刊宗旨和发表范围
Applied and
Environmental Microbiology (AEM) publishes study results that
make significant contributions to applied microbial research, basic
microbial ecology research, and genetic and molecular
investigations of microbial topics of practical value. The journal
sheds new light on key microbiological principles, fundamental
microbial processes, and basic questions in applied and
environmental microbiology. Its original research articles not only
build on our understanding of microbiology but also enable
scientists to advance their own investigations, design new
products, and develop new processes.
Applied and
Environmental Microbiology features:
Commentaries
Minireviews
Meeting Reviews
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The scope of
Applied and Environmental Microbiology covers:
Genetics and Molecular
Enzymology and Protein
Engineering
Physiology
Biotechnology
Public Health
Microbiology
Environmental
Microbiology
Microbial Ecology
Geomicrobiology
Food Microbiology
Plant Microbiology
Invertebrate
Microbiology
Biodegradation
Evolutionary and Genomic
Microbiology
Twice monthly,
with 8,600 allocated pages in 2012. Print ISSN: . Online
AEM has an
Impact Factor of 3.778 and an Eigenfactor score of 0.139
according to the 2010 Journal Citation Reports&. It is
the #1 cited journal in Microbiology and the #1 cited journal in
Biotechnology & Applied Microbiology, with more
than 78,000 citations. AEM is also the #1 journal in Microbiology
and the #2 journal in Biotechnology & Applied
Microbiology ranked by Eigenfactor score.
AEM is ranked
#24 by Impact Factor out of 107 journals in the Microbiology
category as well as #32 out of 160 journals in the Biotechnology
& Applied Microbiology category.
Indexed/Abstracted in: Agricola,
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Scientific Abstracts, Chemical Abstracts Service, Current Contents,
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伍一军在《Applied Microbiology and Biotechnology》上发表最新研究成果
来源：生物通 作者：
摘要: 来自中科院动物研究所的伍一军研究组在《AppliedMicrobiologyandBiotechnology》上发表最新研究成果Afluorescentgeneticallyengineeredmicro-organismthatdegradesorganophosphatesandcommitssuicidewhenrequired。在这些研究领域，分子毒理学研究组已经取得了具有较高学术价值的研究成果，特别是针对OPIDN的研究，跻身该......
专题推荐：
来自中科院动物研究所的伍一军研究组在《Applied&&Microbiology&&and&&Biotechnology》上发表最新研究成果A&&fluorescent&&genetically&&engineered&&micro-organism&&that&&degrades&&organophosphates&&and&&commits&&suicide&&when&&required.&&Applied&&Microbiology&&and&&Biotechnology。
该研究工作得到了国家高技术研究发展计划（863计划）（No.&&；No.&&）及中国科学院动物研究所知识创新工程（KSCX3-IOZ-0701）课题的资助。
文章通讯作者是伍一军教授，分子毒理学研究组组长。
通过重组DNA技术构建的具有特定功能（如：降解农药）的基因工程，即工程微生物&&（genetically&&engineered&&microorganism,&&GEM）具有广阔的应用前景，然而，GEM的应用本身又有可能引发新的生态问题，这种潜在的危险性反过来限制了它的应用。为了研究如何最小化GEM应用的潜在风险，有必要建立用于环境中GEM的安全监测体系和在需要时能减少或消除其存在的主动生物防御（active&&biologic&&containment，ABC）体系。
动物研究所伍一军研究组利用双质粒表达系统成功构建了具有荧光特性和农药降解活性及可诱导性自杀能力的GEM。
在该双质粒表达系统中有两个功能质粒：一个是用于ABC体系的自杀基因双拷贝质粒；另一个是与自杀质粒相容的带有荧光蛋白基因与农药降解酶基因的功能质粒。前者含有来源于Serratia&&marcescens&&的去除前导肽的核酸酶自杀基因，该基因被构建到T7启动子下游，其表达受阿拉伯糖诱导；后者则是带有有机磷水解酶（organophosphorus&&hydrolase，OPH）基因与绿色荧光蛋白（enhanced&&green&&fluorescent&&protein，EGFP）基因的质粒pL-EGFP-OPH，将其与自杀质粒共转于E.&&coli&&BL21AITM&&中得到GEB。
通过荧光检测和酶活性检测以及可诱导性自杀能力的检测，证明该GEB不仅具有可供方便检测的荧光特性，而且还具有可诱导自杀能力，同时还具有较强的农药降解活性。具有自杀控制功能的安全型GEB的研究成功极大地提高了GEM在环境中实际应用的安全性。
安徽桐城人,博士,佐治亚医学院分子医学与遗传学系基因调控专业博士后。现为中科院动物研究所分子毒理学研究组组长，中科院动物研究所研究员，中科院研究生院兼职教授，博士研究生导师。
分子毒理学研究组多年来一直开展化学杀虫剂与生物大分子的相互作用机理及环境毒理学的研究。承担了国家攻关项目、中国科学院重点项目、国家自然科学基金项目、中科院生物特别支持项目、中科院知识创新项目和国际合作项目，并承担中科院创新重大项目和国家863专题项目以及科技部科技支撑项目和863探索项目。先后开展了对一些重要杀虫药剂和神经毒剂的毒性机理的研究，例如：就溴氰菊酯对脑蛋白质磷酸化的影响开展研究，以了解拟除虫菊酯类杀虫剂的神经毒理机制；对有机磷杀虫剂和其他有机磷引起的迟发性神经毒性（&&OPIDN&&）问题开展研究，探索此类毒剂的神经毒性的分子机制；对生物体中重要的代谢酶系&&――&&细胞色素P450酶系的结构、功能开展研究，为这类酶系在农业和环境中的应用打下基础；对杀虫剂和其它农药使用后的转归开展研究，为农产品及其环境的安全性评价提供依据。在这些研究领域，分子毒理学研究组已经取得了具有较高价值的研究成果，特别是针对OPIDN的研究，跻身该领域国际前沿。业已发现，有机磷杀虫剂引起OPIDN的主要靶标蛋白－神经毒性酯酶（NTE）并非神经细胞分化所必需，但却在神经生长发育过程中起着重要的辅助作用。NTE的过表达可以引起细胞周期阻滞。NTE蛋白可通过泛素蛋白酶体途径降解，也可通过溶酶体途径降解，前者与NTE蛋白的调节域(R-)有关；而后者则与NTE的跨膜结构域和调节域(R-)有关。这些研究成果对于了解NTE的生理功能及其在OPIDN中的作用具有重要的意义。
本研究组先后申请国家发明专利2项，编著出版学术著作2部，在国际核心学术刊物上发表研究数十篇。建立了经过中华人民共和国农业部认证的农药残留分析检测实验室。截至2007年底，分子毒理学研究组已培养研究生15人，其中博士研究生8人，硕士研究生7人；现在读研究生8人，已毕业7人。
目前，分子毒理学研究组最感兴趣的研究方向是：(1)有机磷神经毒性的分子机理：NTE的生理功能及其代谢调节；(2)神经毒性杀虫剂与神经毒性化学物对人畜健康危害的控制以及昆虫与其他生物对杀虫药剂耐受性的机理。(3)环境中有毒物的检测及消解：残留农药的非常规检测技术及利用生物工程技术降解残留农药的环境安全问题。
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FEMS MICROBIOL ECOLFEMS MICROBIOLOGY ECOLOGYMICROBIOLOGY33
MICROBIOL-SGMMICROBIOLOGY-SGMMICROBIOLOGY33
BMC MICROBIOLBMC MICROBIOLOGYMICROBIOLOGY33
MICROB ECOLMICROBIAL ECOLOGYMARINE & FRESHWATER BIOLOGY23
MICROB ECOLMICROBIAL ECOLOGYECOLOGY33
MICROB ECOLMICROBIAL ECOLOGYMICROBIOLOGY33
INT MICROBIOLINTERNATIONAL MICROBIOLOGYBIOTECHNOLOGY & APPLIED MICROBIOLOGY33
INT MICROBIOLINTERNATIONAL MICROBIOLOGYMICROBIOLOGY33
SYST APPL MICROBIOLSYSTEMATIC AND APPLIED MICROBIOLOGYBIOTECHNOLOGY & APPLIED MICROBIOLOGY33
SYST APPL MICROBIOLSYSTEMATIC AND APPLIED MICROBIOLOGYMICROBIOLOGY33
BIOMICROFLUIDICSBiomicrofluidicsNANOSCIENCE & NANOTECHNOLOGY33
BIOMICROFLUIDICSBiomicrofluidicsBIOPHYSICS43
J MOL MICROB BIOTECHJOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGYBIOTECHNOLOGY & APPLIED MICROBIOLOGY33
J MOL MICROB BIOTECHJOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGYMICROBIOLOGY43
RES MICROBIOLRESEARCH IN MICROBIOLOGYMICROBIOLOGY43
J APPL MICROBIOLJOURNAL OF APPLIED MICROBIOLOGYBIOTECHNOLOGY & APPLIED MICROBIOLOGY33
J APPL MICROBIOLJOURNAL OF APPLIED MICROBIOLOGYMICROBIOLOGY43
J MICROBIOL METHJOURNAL OF MICROBIOLOGICAL METHODSBIOCHEMICAL RESEARCH METHODS43
J MICROBIOL METHJOURNAL OF MICROBIOLOGICAL METHODSMICROBIOLOGY43
INT J SYST EVOL MICRINTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGYMICROBIOLOGY43
FEMS MICROBIOL LETTFEMS MICROBIOLOGY LETTERSMICROBIOLOGY44
ENZYME MICROB TECHENZYME AND MICROBIAL TECHNOLOGYBIOTECHNOLOGY & APPLIED MICROBIOLOGY44
ARCH MICROBIOLARCHIVES OF MICROBIOLOGYMICROBIOLOGY44
ADV APPL MICROBIOLADVANCES IN APPLIED MICROBIOLOGYBIOTECHNOLOGY & APPLIED MICROBIOLOGY44
ADV APPL MICROBIOLADVANCES IN APPLIED MICROBIOLOGYMICROBIOLOGY44
J EUKARYOT MICROBIOLJOURNAL OF EUKARYOTIC MICROBIOLOGYMICROBIOLOGY44
J MICROBIOLJOURNAL OF MICROBIOLOGYMICROBIOLOGY44
MICROBIOL RESMICROBIOLOGICAL RESEARCHMICROBIOLOGY44
FUTURE MICROBIOLFuture MicrobiologyMICROBIOLOGY44
CAN J MICROBIOLCANADIAN JOURNAL OF MICROBIOLOGYIMMUNOLOGY44
CAN J MICROBIOLCANADIAN JOURNAL OF MICROBIOLOGYBIOCHEMISTRY & MOLECULAR BIOLOGY44
CAN J MICROBIOLCANADIAN JOURNAL OF MICROBIOLOGYBIOTECHNOLOGY & APPLIED MICROBIOLOGY44
CAN J MICROBIOLCANADIAN JOURNAL OF MICROBIOLOGYMICROBIOLOGY44
CURR MICROBIOLCURRENT MICROBIOLOGYMICROBIOLOGY44
XJ BASIC MICROBJOURNAL OF BASIC MICROBIOLOGYMICROBIOLOGY44
NEW MICROBIOLNew MicrobiologicaMICROBIOLOGY44
XREV MED MICROBIOLREVIEWS IN MEDICAL MICROBIOLOGYMICROBIOLOGY44
J GEN APPL MICROBIOLJOURNAL OF GENERAL AND APPLIED MICROBIOLOGYBIOTECHNOLOGY & APPLIED MICROBIOLOGY44
J GEN APPL MICROBIOLJOURNAL OF GENERAL AND APPLIED MICROBIOLOGYMICROBIOLOGY44
MICROBIOLOGY+MICROBIOLOGYMICROBIOLOGY44
METHOD MICROBIOLMETHODS IN MICROBIOLOGYBIOCHEMICAL RESEARCH METHODS44
METHOD MICROBIOLMETHODS IN MICROBIOLOGYMICROBIOLOGY44
J RAPID METH AUT MICJOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGYBIOTECHNOLOGY & APPLIED MICROBIOLOGY44
J RAPID METH AUT MICJOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGYFOOD SCIENCE & TECHNOLOGY44
APPL BIOCHEM MICRO+APPLIED BIOCHEMISTRY AND MICROBIOLOGYBIOTECHNOLOGY & APPLIED MICROBIOLOGY44
APPL BIOCHEM MICRO+APPLIED BIOCHEMISTRY AND MICROBIOLOGYMICROBIOLOGY44
XJ MICROPALAEONTOLJOURNAL OF MICROPALAEONTOLOGYPALEONTOLOGY44
AFR J MICROBIOL RESAfrican Journal of Microbiology ResearchMICROBIOLOGY44
ANN MICROBIOLANNALS OF MICROBIOLOGYBIOTECHNOLOGY & APPLIED MICROBIOLOGY44
ANN MICROBIOLANNALS OF MICROBIOLOGYMICROBIOLOGY44
BRAZ J MICROBIOLBRAZILIAN JOURNAL OF MICROBIOLOGYMICROBIOLOGY44
SUPERLATTICE MICROSTSUPERLATTICES AND MICROSTRUCTURESPHYSICS, CONDENSED MATTER44
CLIN MICROBIOL REVCLINICAL MICROBIOLOGY REVIEWSMICROBIOLOGY11
XCURR TOP MICROBIOLCURRENT TOPICS IN MICROBIOLOGY AND IMMUNOLOGYIMMUNOLOGY22
XCURR TOP MICROBIOLCURRENT TOPICS IN MICROBIOLOGY AND IMMUNOLOGYMICROBIOLOGY22
ANTIMICROB AGENTS CHANTIMICROBIAL AGENTS AND CHEMOTHERAPYMICROBIOLOGY22
ANTIMICROB AGENTS CHANTIMICROBIAL AGENTS AND CHEMOTHERAPYPHARMACOLOGY & PHARMACY22
J ANTIMICROB CHEMOTHJOURNAL OF ANTIMICROBIAL CHEMOTHERAPYINFECTIOUS DISEASES22
J ANTIMICROB CHEMOTHJOURNAL OF ANTIMICROBIAL CHEMOTHERAPYMICROBIOLOGY22
J ANTIMICROB CHEMOTHJOURNAL OF ANTIMICROBIAL CHEMOTHERAPYPHARMACOLOGY & PHARMACY22
J CLIN MICROBIOLJOURNAL OF CLINICAL MICROBIOLOGYMICROBIOLOGY32
XCLIN MICROBIOL INFECCLINICAL MICROBIOLOGY AND INFECTIONINFECTIOUS DISEASES23
XCLIN MICROBIOL INFECCLINICAL MICROBIOLOGY AND INFECTIONMICROBIOLOGY33
INT J MED MICROBIOLINTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGYVIROLOGY33
INT J MED MICROBIOLINTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGYMICROBIOLOGY33
MICROBES INFECTMICROBES AND INFECTIONIMMUNOLOGY33
MICROBES INFECTMICROBES AND INFECTIONMICROBIOLOGY33
MICROBES INFECTMICROBES AND INFECTIONVIROLOGY43
MICROVASC RESMICROVASCULAR RESEARCHPERIPHERAL VASCULAR DISEASE33
MICROCIRCULATIONMICROCIRCULATIONPERIPHERAL VASCULAR DISEASE33
MICROCIRCULATIONMICROCIRCULATIONHEMATOLOGY33
INT J ANTIMICROB AGINTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTSINFECTIOUS DISEASES33
INT J ANTIMICROB AGINTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTSMICROBIOLOGY33
INT J ANTIMICROB AGINTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTSPHARMACOLOGY & PHARMACY33
EUR J CLIN MICROBIOLEUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASESINFECTIOUS DISEASES33
EUR J CLIN MICROBIOLEUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASESMICROBIOLOGY33
DIAGN MICR INFEC DISDIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASEINFECTIOUS DISEASES33
DIAGN MICR INFEC DISDIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASEMICROBIOLOGY33
MICROB PATHOGENESISMICROBIAL PATHOGENESISIMMUNOLOGY43
MICROB PATHOGENESISMICROBIAL PATHOGENESISMICROBIOLOGY43
J MED MICROBIOLJOURNAL OF MEDICAL MICROBIOLOGYMICROBIOLOGY43
FEMS IMMUNOL MED MICFEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGYINFECTIOUS DISEASES44
FEMS IMMUNOL MED MICFEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGYIMMUNOLOGY44
FEMS IMMUNOL MED MICFEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGYMICROBIOLOGY44
ORAL MICROBIOL IMMUNORAL MICROBIOLOGY AND IMMUNOLOGYDENTISTRY, ORAL SURGERY & MEDICINE24
ORAL MICROBIOL IMMUNORAL MICROBIOLOGY AND IMMUNOLOGYIMMUNOLOGY44
ORAL MICROBIOL IMMUNORAL MICROBIOLOGY AND IMMUNOLOGYMICROBIOLOGY44
MED MICROBIOL IMMUNMEDICAL MICROBIOLOGY AND IMMUNOLOGYIMMUNOLOGY44
MED MICROBIOL IMMUNMEDICAL MICROBIOLOGY AND IMMUNOLOGYMICROBIOLOGY44
MICROB DRUG RESISTMICROBIAL DRUG RESISTANCE-MECHANISMS EPIDEMIOLOGY AND DISEASEINFECTIOUS DISEASES44
MICROB DRUG RESISTMICROBIAL DRUG RESISTANCE-MECHANISMS EPIDEMIOLOGY AND DISEASEMICROBIOLOGY44
MICROB DRUG RESISTMICROBIAL DRUG RESISTANCE-MECHANISMS EPIDEMIOLOGY AND DISEASEPHARMACOLOGY & PHARMACY44
XMICROSC RES TECHNIQMICROSCOPY RESEARCH AND TECHNIQUEANATOMY & MORPHOLOGY34
XMICROSC RES TECHNIQMICROSCOPY RESEARCH AND TECHNIQUEBIOLOGY34
XMICROSC RES TECHNIQMICROSCOPY RESEARCH AND TECHNIQUEMICROSCOPY34
COMP IMMUNOL MICROBCOMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASESVETERINARY SCIENCES24
COMP IMMUNOL MICROBCOMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASESIMMUNOLOGY44
COMP IMMUNOL MICROBCOMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASESMICROBIOLOGY44
MICROBIOL IMMUNOLMICROBIOLOGY AND IMMUNOLOGYIMMUNOLOGY44
MICROBIOL IMMUNOLMICROBIOLOGY AND IMMUNOLOGYMICROBIOLOGY44
CLIN HEMORHEOL MICROCLINICAL HEMORHEOLOGY AND MICROCIRCULATIONPERIPHERAL VASCULAR DISEASE44
CLIN HEMORHEOL MICROCLINICAL HEMORHEOLOGY AND MICROCIRCULATIONHEMATOLOGY44
XENFERM INFEC MICR CLENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICAINFECTIOUS DISEASES44
XENFERM INFEC MICR CLENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICAMICROBIOLOGY44
MICROSURGMICROSURGERYSURGERY44
XJ RECONSTR MICROSURGJOURNAL OF RECONSTRUCTIVE MICROSURGERYSURGERY44
注：本目录分类来源于中国科学院，参考“期刊印证报告”（JCR），所收集信息仅供科学研究使用。
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