后使用快捷导航没有帐号？
请完成以下验证码
安装autoform许可证时
电脑服务里没有Autoform
系统win10& & 64位的& & 装的是R6的
防火墙啊& &360啊& &都关了的
width:100%">
用的是不是tianyi的破解，仔细看安装说明，然后重启试试
width:100%">
用的是不是tianyi的破解，仔细看安装说明，然后重启试试
许可证装都装不上& &
何来的破解之说& & 不都是装了在破解的嘛！
width:100%">
有那么复杂么？装不上 证书，就找个别人装好破解证书的电脑，拷贝到指定目录。。然后手动起动rlm.exe&&和服务项 防火墙半毛钱关系没有。。。
width:100%">
最后说一点，这个问题主要是出现在R6版本，R5.2 R7都不存在该问题。既然你软件不熟，就更新到最新版本学，或者用低一点版本的。
如果你对R4以后的版本很熟了，建议你用R7真的很好用。。更加人性化了。。这个软件参数少，自己用起来还是比较简单的。属于傻瓜软件。
width:100%">
各位大师，有R7版本的学习资料吗？有的话分享下
width:100%">
各位中国成型论坛的网友，I am back，大家都知道每次我回来，都会给大家带好东西，当
求大神帮忙
DF593 安装完运行异常
本次测试对比三款软件的修边线展开能力，其中包括：AutoForm-Geometrical-Flange、Aut
汽车覆盖件冲压工艺设计
**** 本内容被作者隐藏 ****
各位大神，求助一下，WIN10 适合安装哪个版本的AUTOFORM啊， 我下载的 R5.2按照教程安
怎么成型？？？
周一至周日 8:30-20:30
关注新浪微博
关注官方微信FirstChoice& RLM-RACE Kit
FirstChoice& RLM-RACE Kit
Invitrogen&
&相关应用：
内容加载时出错！
选择定价方案
单价&({{currency}})
{{product.sku}}
也被称为 {{product.formattedSku}}&
{{plan.description}}
{{product.availability.message}}
{{currency}}
Save to list
Create your first list
{{addItemToList.successMessage}}
您所选商品的库存
0" ng-repeat="scProduct in supplyCenter.scProducts">
{{supplyCenter.scName}}({{scProduct.stockOnHand}} 现货供应)
{{supplyCenter.scName}}(缺货)
{{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
The FirstChoice& RLM-RACE Kit is designed to amplify cDNA only from full-length, capped mRNA, usually producing a single band after PCR. This kit is a major improvement over the basic rapid amplification of cDNA ends (RACE) protocol. The RLM-RACE procedure selects only full-length mRNA&no rRNA, tRNA or degraded RNA&and facilitates the cloning of sequences from the 5' ends of messages.Features of the FirstChoice& RLM-RACE Kit:& From RNA to PCR product in less than a day& Yields single, specific product from rare transcripts& Selects 5' and/or 3' ends of true messages& Efficient&all enzymatic reactions are optimized to ensure detection of even the rarest mRNARACERapid amplification of cDNA ends (5'-RACE) is a polymerase chain reaction-based technique developed to facilitate the cloning of sequence from the 5'-ends of messages. The FirstChoice& RLM-RACE Kit is a major improvement to the basic RACE protocol.Selects full-length mRNA&no rRNA, tRNA or degraded mRNAIn the FirstChoice& RLM-RACE procedure, full-length mRNAs are selected by treating total or poly(A) RNA with calf intestinal phosphatase (CIP) to remove the 5'-phosphate from all molecules which contain free 5'-phosphates (ribosomal RNA, fragmented mRNA, tRNA, and contaminating genomic DNA). Full-length mRNAs are unaffected. The RNA is then treated with tobacco acid pyrophosphatase (TAP) to remove the cap structure from the full-length mRNA leaving a 5'-monophosphate. A synthetic RNA adapter is ligated to the RNA population&only molecules containing a 5'-phosphate, the uncapped, full-length mRNAs, will accept the adapter. Random-primed, reverse transcription reactions and nested PCR are then performed to amplify the 5'-end of your specific transcript.From RNA to PCR in less than a dayEach step of the FirstChoice& RLM-RACE procedure has been optimized, so you can complete all the enzymatic reactions and even start PCR in no more than 5 hours. The FirstChoice& RLM-RACE Kit contains reagents and enzymes to produce 5 RLM-RACE-ready cDNA preparations, in addition to primers and nested RACE adapter primers for 100 PCR reactions. Reverse transcription reagents are also included. Each kit contains control RNA and primers to test the kit's performance along with a detailed Instruction Manual. SuperTaq& Thermostable Taq DNA Polymerase is available separately. For optimal amplification of fragments &1 kb, use SuperTaq-Plus. Also included are a 3' RACE Adapter and Primers for 3' RACE.
For Research Use Only. Not for use in diagnostic procedures.
5' FirstChoice(TM) RLM-RACE for mouse CXCR4 gene and Xenopus TGF-beta-related gene.
Final Product:
Final Product Size(s):
7 kb or less
Optimal Reaction Temperature:
Product Line:
Ambion&,&FirstChoice&
Product Size:
Reaction Format:
Separate Components
Reverse Transcriptase:
Sample Type (General):
Shipping Condition:
内容及储存
The following components should be stored at &20&C:
Calf Intestine Alkaline Phosphatase
10X CIP Buffer
Tobacco Acid Pyrophosphatase
10X TAP Buffer, T4 RNA Ligase
10X T4 RNA Ligase Buffer
5' RACE Adapter
3' RACE Adapter
5' RACE Outer Primer
5' RACE Inner Primer
3' RACE Outer Primer
3' RACE Inner Primer
Ammonium Acetate Stop Solution
M-MLV Reverse Transcriptase
10X RT Buffer
10X PCR Buffer
Random Decamers
RNase Inhibitor
Mouse Thymus RNA
5' RACE Outer Control Primer
5' RACE Inner Control Primer
5' PCR Control Primer
3' RACE Control Primer
Nuclease-free water may be stored at any temperature.
0 || results.spectraList.length > 0">
0 || results.protocolList.length > 0">
手册和实验方案
0 || results.lullList.length > 0">
0" id="pdp-results-faqs">
0" id="pdp-results-citations">
100 reactions
CNY&2,268.00
添加至收藏夹
25 reactions
CNY&7,055.00
添加至收藏夹
CNY&671.00
添加至收藏夹
Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
赛默飞世尔科技（中国）有限公司地址(中国总部)：上海市浦东新区新金桥路27号3& 6& 7号楼
电话：86-21-YY帐号登录
登录前请仔细阅读